EUROLAB, with its state-of-the-art accredited laboratories and expert team, offers precise and rapid testing services within the scope of ISO 29701 testing. ISO 29701 describes the application of an assay using the Limulus amebocyte lysate (LAL) reagent for the evaluation of nanomaterials for cell-based in vitro biological testing systems.
The test is suitable for use in such media with nanomaterial samples dispersed in aqueous media, for example, water, serum or reaction medium, and incubated with the nanomaterials for an appropriate period of time at 37°C.
ISO 29701 is limited to test samples for in vitro systems, but the methods can also be adapted to nanomaterials to be administered to animals by parenteral routes.
For the purpose of toxicity screening or biocompatibility testing of nanomaterials or mechanism studies of possible toxicity caused by nanomaterials, various cell-based in vitro test systems and in vivo animal models are being developed and used.
In in vitro testing systems, macrophages and other relevant mammalian cells are frequently used as test cells, especially for nanomaterials, because they are primarily the responsible surveillance cells in the body. However, these cells are highly reactive to endotoxins; therefore, it is difficult to separate the response to endotoxins from the response to nanomaterials. As a result, contamination from endotoxins will confound the results of in vitro tests.
Contamination of test samples with endotoxins can be reduced if appropriate precautions are taken in test sample preparation. Therefore, preliminary detection of endotoxins is necessary to minimize contamination with endotoxins or to confirm insignificant endotoxin levels in the test sample. Measurement of endotoxin levels is also important for adequate interpretation of data obtained from in vitro biological testing systems.
EUROLAB assists manufacturers with ISO 29701 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.
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EUROLABreporting and certification activities, United Accreditation Foundation (UAF), Turkish Accreditation Agency (TÜRKAK) ve Enterprise Accreditation Foundation (EAF) based on the accreditation provided by the accreditation body. The Enterprise Accreditation Foundation (EAF) accreditation body is also a signatory of the United Nations (UN GLOBAL COMPACT) organization.
