Diagnostic Nucleic Acid Microarrays address sequence-based detection of variations in DNA sequence and analysis of gene expression. Hereditary variations, somatic changes, methylation profiling, pathogen profiling including antibiotic resistance analysis, expression profiling, and gene dosage genomic hybridization provide recommendations for many aspects of the microarray process. These include: an overview of a method; nucleic acid extraction; provides the preparation, processing and evaluation of genetic material, interpretation and reporting of results.
Diagnostic Microarrays Diagnostic nucleic acid microarrays are a relatively new outcome of more traditional molecular diagnostic methods. Nucleic acid-based microarrays also have diagnostic potential for the identification of infectious disease organisms from a variety of sample matrices. Other configurations of microarrays enable comparative studies of gene expression in selected tissues and samples, resulting in diagnosis and response predictions. These devices represent multiplexed analysis of clinical samples and have unique manufacturing and quality control concerns, analytical and clinical validation differences compared to simple unitary tests, and novel interpretation algorithms.
These tests can complement or replace more traditional diagnostic methods and in some cases may be the only available approach to diagnosis. The utility of diagnostic microarrays includes predicting disease susceptibility, identifying pathological organisms, screening for carriers of recessive traits, performing prenatal diagnosis, making earlier or more reliable diagnosis of cancer, classifying tissues and tumors by molecular signature, and making treatment decisions based on polymorphic markers.
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