Phi X174 Standard Test for Bacteriophage Prohead Isolation and Identification

Medical Tests

Phi X174 Standard Test for Bacteriophage Prohead Isolation and Identification

EUROLAB laboratory provides testing and compliance services within the scope of Phi X174 standard. The morphogenesis of the bacteriophage phi X174 was investigated using an in vitro DNA expression system. A B-mutant-infected cell extract is capable of synthesizing infectious phage in vitro when B gene function is achieved by adding an ammonium sulfate fraction of a C-mutant-infected cell extract.

Phi X174 Standard Test for Bacteriophage Prohead Isolation and Identification

This fraction contains omega complex, which is a complex of S=108 and phage-encoded proteins; No B-mutant extract. The purified omega complex isolated from the C-mutant extract caused the synthesis and encapsulation of viral DNA when added to the B-mutant extract.

The omega complex contains the B protein, but it is the intact omega complex that functions in the in vitro complement of the B mutant extract as other fractions containing the B protein but not the omega complex have little or no complementary activity.

The results show that the omega complex is phi X174 prohead. The B protein is not found in 132S or 114S phage particles, but is an essential component of procephalon. This suggests that it may have a scaffolding function.

EUROLAB assists manufacturers with Phi X174 testing compatibility. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.

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