Determination of absorbance The absorbance of a solution is defined as the base 10 logarithm of the inverse of the transmission for monochromatic radiation.
In the absence of other physico-chemical factors, the absorbance is proportional to the path length of the radiation and the concentration of the substance in the solution according to the equation.
Unless otherwise specified, measure absorbance at the specified wavelength using a path length of 1 cm. Unless otherwise specified, measurements are made with reference to the same solvent measured against air and should not exceed 0,4 at the prescribed wavelength and preferably less than 0,2. Plot the absorbance as ordinate with respect to wavelength or as a function of wavelength as abscissa.
When a monograph gives a single value for the location of an absorption maximum, it is understood that the value obtained cannot differ by more than ± 2 nm.
The apparatus suitable for measuring in the ultraviolet and visible range of the spectrum consists of spectrophotometers, an optical system capable of producing monochromatic radiation in the range of 200-800 nm, and a device suitable for absorbance measurement.
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