OECD 431 OECD Guidelines for the Testing of Chemicals, In Vitro Skin Corrosion, Reconstructed Human Epidermis (RHE) Test Method

EUROLAB laboratory provides testing and compliance services within the scope of OECD 431 standard. The OECD chemicals testing guidelines are a collection of approximately 150 of the most relevant internationally recognized test methods used by government, industry and independent laboratories to identify and characterize the potential hazards of chemicals.

They are a set of tools for professionals used primarily in regulatory safety testing and then in chemical and chemical product declaration, chemical registration and chemical evaluation. They can also be used for selection and sequencing of candidate chemicals during the development of new chemicals and products and in toxicology research. This set of tests covers health effects.

The test described in this Test Guide allows the identification of corrosive chemicals and mixtures and allows the identification of non-corrosive substances and mixtures when supported by a weight of evidence determination using other available information.

The test protocol can also provide an indication of the distinction between serious and less severe skin abrasives. This Test Guide does not require the use of live animals or animal tissue for assessment of skin abrasiveness.

The test material (solid or liquid) is applied homogeneously and topically to a three-dimensional model of human skin containing a functional stratum corneum and at least a reconstructed epidermis. Two tissue replicates are used for each treatment (exposure time) and controls.

Corrosive materials are defined by their ability to produce a reduction in cell viability below defined threshold levels at certain exposure times. Colored chemicals can also be tested using an HPLC procedure. The principle of the human skin model assay is based on the hypothesis that corrosive chemicals can penetrate the stratum corneum by diffusion or erosion and are cytotoxic to the underlying cell layers.

EUROLAB assists manufacturers with OECD 431 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.