Gas Chromatography Mass Spectrometry (GC-MS / MS) Test Laboratory

Chemical Tests

Gas Chromatography Mass Spectrometry (GC-MS / MS) Test Laboratory

Gas chromatography (GC, gas chromatography) is an analytical technique used to separate the chemical components of a sample mixture and then determine their presence or absence or how much they are present. These chemical components are usually organic molecules or gases. In order to be successful in gas chromatography analyzes, the molecular weights of these components should generally be 1250 g / mol and below, volatile and thermally stable.

Gas Chromatography Mass Spectrometry (GC-MS / MS) Test Laboratory

Gas chromatography is a technique widely used in many industries. For example, this technique is used for quality control in the production activities of many products, from cars to chemicals and pharmaceuticals, for research purposes from the analysis of meteorites to natural products, and for safety from the environment to food and forensics. Gas chromatography is mainly used in combination with mass spectrometry to enable the identification of chemical components.

While gas chromatography is running, it uses a carrier gas in the separation processes, which is effective in the mobile phase part. The carrier gas transports sample molecules through the gas chromatography system, ideally without reacting with the sample or damaging the instrument components. The sample is first transferred from an autosampler and entered into the gas chromatograph. The sample is injected into the gas chromatograph inlet through a channel that allows the injection of the sample mixture without losing the mobile phase. The analytical column connected to the inlet is a tall, narrow, fused silica or metal tube containing the stationary phase coated on the inner walls. The analytical column is kept in a heated column oven to separate the less volatile components during analysis. At the exit of the column is a detector that responds to chemical components separated from the column to generate a signal. The signal is recorded on a computer.

Mass spectrometry is an analytical technique added to a gas chromatograph and used instead of a gas chromatography detector. Neutral molecules are separated from the analytical column and ionized at the ion source to produce molecular ions. The fragment and molecular ions are then separated and detected on the mass analyzer based on their mass / charge ratio.

Data from a gas chromatography mass spectrometer are three-dimensional. Authentication provides mass spectra used to identify unknown analytes and determine structural and chemical properties of molecules, as well as chromatogram used for qualitative and quantitative analysis.

From the chromatogram a lot of information is obtained about the health of the gas chromatography or gas chromatography mass spectrometry system, as well as the data required for qualitative or quantitative analysis.

Compared to other separation techniques, gas chromatography has a high capacity with its ability to separate hundreds of compounds. However, for some applications where thousands of peaks need to be separated, there are not enough plates to separate them all chromatographically. For example, diesel analysis, detection of trace analytes in environmentally complex matrices, biological analysis or food analysis.

Spectral resolution with which a mass spectrometer is paired with a gas chromatograph allows analysis to be done without full chromatographic resolution, but for this to be fully successful, the pairing peaks must have different spectra.

Gas chromatography is used to analyze thousands of compounds in many different matrices, from solids to gases. It is a robust technique and can easily match other techniques including mass spectrometry. However, gas chromatography is limited to analyzing volatile compounds from helium or hydrogen to about 1250 molecular weights. Also, thermally labile compounds can decompose in a hot gas chromatography. Cold injection techniques and low temperatures should be used to minimize this.

The most common problem with gas chromatography is leaks. The mobile phase is a gas and flows through the system, so proper fitting of parts and consumables is important as well as regular leak control. In addition, gas chromatography is another problem, especially for analytes that are at trace levels. Glass liners and dirt build-up in the system cause peaks, irreversible adsorption or catalytic degradation. The inlet is the most problematic area, where the sample is injected, evaporated and transferred to the gas chromatography column. That's why regular intake maintenance is essential along with the use of the correct consumables.

Our organization provides gas chromatography mass spectrometry (GC-MS / MS) test laboratory services to enterprises that demand, within the framework of national and international standards, with a trained and expert staff and advanced technological equipment, among numerous testing, measurement, analysis and evaluation studies.

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