EUROLAB, with its state-of-the-art accredited laboratories and expert team, provides precise and fast testing services within the scope of ISO 12966-1 testing. This standard provides an overview of the gas chromatographic determination of free and bound fatty acids in animal and vegetable fats and oils after their conversion to fatty acid methyl esters (FAMEs).
The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and oils or fatty foodstuffs after removal of fats from the matrix.
Depending on the bound fatty acids of triacylglycerols (TAGs) and the esterification method, free fatty acids (FFA) and other lipids are converted to fatty acid methyl esters (FAMEs) determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically, more than 50 different fatty acids may be present) capillary columns from 10 m to 100 m long are used for separation.
The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols, fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards, FAMEs from C4 to C26 can be specified, including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. For the determination of short chain fatty acids, isopropyl and butyl esters are often used to avoid interference with the solvent peak and reduce differences in detector responses.
EUROLAB assists manufacturers with ISO 12966-1 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.
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