OECD 236 Testing of Chemicals - Fish Embryo Acute Toxicity (FET) Test

EUROLAB, together with its state-of-the-art accredited laboratories and expert team, provides precise and rapid testing services within the scope of OECD 236 testing. The test method described in this test guide is intended to determine the acute or fatal toxicity of chemicals in the embryonic stages of fish (Danio rerio).

Newly fertilized zebrafish eggs are exposed to the test chemical for a period of 96 hours. Every 24 hours. Twenty embryos (one embryo per well) are exposed to the chemical tested at each concentration level. The test includes five increasing concentrations of the tested chemical and a control.

Four apical observations are recorded every 24 hours as indicators of lethality:

• Coagulation of fertilized eggs;
• Absence of somite formation;
• Inability of the tail bud to separate from the yolk;
• Lack of heartbeat.

At the end of the exposure period, acute toxicity is determined based on a positive result in any of the four recorded apical observations and the LC50 is calculated.

The OECD guidelines for testing chemicals are a collection of approximately 150 of the most relevant internationally recognized test methods used by government, industry and independent laboratories to identify and characterize the potential hazards of chemicals.

EUROLAB assists manufacturers with OECD 236 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.