EUROLAB, with its state-of-the-art accredited laboratories and expert team, provides precise and fast testing services within the scope of ISO 12966-2 testing. ISO 12966-2 specifies methods for preparing methyl esters of fatty acids. Includes methods for the preparation of fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps.
Four methylation methods are specified to meet different requirements, namely:
The methyl esters thus produced are used in a variety of analytical procedures that require derivatives, for example gas-liquid chromatography (GLC), thin layer chromatography (TLC), and infrared spectrometry (IR). Milk and dairy products (or fats derived from milk and dairy products) are outside the scope of this document.
This method is applicable to the routine analysis of edible fats and oils containing fatty acids up to butanoic acid and/or the determination of butanoic acid or hexanoic acid by GC using an internal standard.
Alkaline catalysts transesterify neutral lipids in the presence of anhydrous methanol (transmethylation) faster than acid catalysts. The disadvantages of such alkali-catalyzed procedures are that the free fatty acids are not esterified and the presence of water may prevent completion of transmethylation (hydrolysis of FAMEs to free fatty acids). The most commonly used reagents are potassium and sodium hydroxide and sodium methoxide in the presence of anhydrous methanol.
EUROLAB assists manufacturers with ISO 12966-2 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.
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