This part of the ISO 6888-1 standard developed by the International Organization for Standardization (ISO) applies to products intended for human consumption or animal feeding, by counting colonies obtained in an intact medium (Baird-Parker medium) after aerobic incubation at 35 °C or 37 °C. Specifies a horizontal method for enumeration of coagulation-positive staphylococci.
-numaralandirmasi-icin-yatay-yontem---bolum-1---baird-parker-agar-ortamini-kullanma-teknigi.jpg "ISO 6888-1 Horizontal Method for Enumeration of Coagulation-Positive Staphylococci (Staphylococcus Aureus And Other Species) - Part 1 - Technique Using Baird-Parker Agar Media")
Due to the wide variety of food and feed products, this horizontal method may not be suitable in every detail for some products. In this case, different methods specific to these products can be used if absolutely necessary for legitimate technical reasons. However, every attempt should be made to apply this horizontal method as much as possible.
A later review of this section of ISO 6888 will take into account all available information regarding the extent to which this horizontal method was followed and, in the case of certain products, the reasons for deviations from it.
Harmonization of test methods cannot be immediate and International Standards and national standards that are not compatible with this horizontal method may already exist for certain product groups. When such standards are reviewed, it is hoped that they will be modified to conform to this part of ISO 6888 so that eventually the only remaining deviations from this horizontal method will be those necessary for well-known technical reasons.
It is important to inoculate the surface of the solid selective culture medium using double plates with a certain amount of initial suspension if the product is liquid, or a certain amount of test sample in the case of other products.
Inoculation should be made using decimal dilutions of the test sample or initial suspension with two plates per dilution under the same conditions.
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