Liquid Chromatography Mass Spectrometry (LC-MS / MS) Test Laboratory

Liquid chromatography technique (LC, liquid chromatography) is a technique used to separate a sample into individual parts. This separation takes place depending on the interactions of the sample with mobile and stationary phases. There are many stationary or mobile phase combinations used to separate a mixture. Therefore, there are several different types of chromatography that classify phases according to their physical state.

Chromatography methods are a technique performed in vitro to separate mixtures into components and include simple or complex methods. There are many different types of chromatography (separation) from paper chromatography and thin layer chromatography to gas chromatography. Among these, liquid-solid column chromatography is the most popular. This technique has a liquid mobile phase which brings with it components which are slowly filtered off from the solid stationary phase.

Components contained in a mixture are divided into a column according to the affinity of each component for the mobile phase. In this way, if the components have different polarities and a mobile phase with a different polarity is passed through the column, one component moves faster through the column than the other. Since molecules of the same compound usually move in groups, the compounds are divided into different bands within the column. These bands can be seen if the separated components are colored. If it is not colored, the presence of bands is detected by other analysis techniques such as ultraviolet spectroscopy.

Mass spectrometry is a useful analytical tool used to measure the mass-to-charge ratio of one or more molecules in a sample. These measurements are often used to calculate the exact molecular weight of sample components. In general, mass spectrometers are used to identify unknown compounds through molecular weight determination, to measure known compounds, and to determine the structure and chemical properties of molecules.

Each mass spectrometer consists of at least three components: ionization source, mass analyzer, and ion detection system. Molecules are converted into gas phase ions to be moved and manipulated by external electric and magnetic fields. Once ionized, the ions are grouped and separated according to their mass-to-charge ratio. There are several mass analyzers today, each with adjustments for processing speed, separation resolution, and other operational requirements. The separated ions are then measured and sent to a data system where they are stored along with their mass-to-charge ratios. A mass spectrum is the mass-to-charge ratio of the ions in a sample drawn according to their densities.

Liquid chromatography mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry. These combined systems are very common in chemical analysis because both techniques have individual capabilities. While liquid chromatography separates mixtures with multiple components, mass spectrometry ensures the structural identity of the individual components with high molecular specificity and detection sensitivity. These two techniques are used to analyze organic, inorganic and biochemical compounds commonly found in complex samples of environmental and biological origin. Therefore, liquid chromatography is applied in a wide variety of industries, including mass spectrometry, environmental monitoring, food processing, pharmaceutical, agrochemical, biotechnology and cosmetics.

Linking mass spectrometry to chromatographic techniques is always preferred due to the sensitive and highly specific nature of mass spectrometry.

Advanced laboratories use a wide variety of mass spectrometry methods to obtain the most precise mass spectral data from all types of analytes such as gases, liquids, solids, and more. These laboratories recommend businesses the correct mass spectrometric technique for sample types. In this way, unknown species are identified and measured in analytical chromatography.

Our organization has become a reliable partner for its customers in various industries. Developing a unique expertise in the field of laboratory testing services, our organization is constantly increasing its capabilities with new analytical tools and techniques. Numerous different mass spectrometry methods are applied in our organization and the chemical composition of the samples is determined with higher resolution than ever before.

The best way to identify the unknowns is liquid chromatography mass spectroscopy method. This method is particularly superior in identifying ionizable species. Reliable and accurate quantitation is achieved at the level of parts per billion.

Our organization provides liquid chromatography mass spectrometry (LC-MS / MS) test laboratory services to demanding enterprises within the framework of national and international standards, with a trained and expert staff and advanced technological equipment, among numerous testing, measurement, analysis and evaluation studies.