ISO 16649-2 Horizontal Method Test Standard for Counting Beta-Glucuronidase Positive Escherichia Coli

Chemical Tests

ISO 16649-2 Horizontal Method Test Standard for Counting Beta-Glucuronidase Positive Escherichia Coli

EUROLAB laboratory provides testing and compliance services within the scope of ISO 16649-2 standard. This part of the ISO 16649 standard specifies a horizontal method for counting β-glucuronidase positive Escherichia coli in products intended for human consumption or animal feed. It uses a colony count technique at 44°C on a solid medium containing a chromogenic component for the detection of the β-glucuronidase enzyme.

ISO 16649-2 Horizontal Method Test Standard for Counting Beta-Glucuronidase Positive Escherichia Coli

Due to the wide variety of food and feed products, this horizontal method may not be suitable for some products in every detail. In this case, different methods specific to these products may be used if absolutely necessary for legitimate technical reasons. However, every attempt should be made to apply this horizontal method as much as possible.

A later review of this part of ISO 16649 will consider all available information on the extent to which this horizontal method has been followed and, in the case of certain products, the reasons for deviations from it.

Harmonization of test methods cannot be immediate and International or national standards may already exist for certain product groups that are not compatible with this horizontal method. When such standards are reviewed, it is hoped that they will be modified to conform to this part of ISO 16649 so that eventually the only remaining deviations from this horizontal method will be those necessary for well-known technical reasons.

Escherichia coli strains that do not grow at 44°C and especially those that are β-glucuronidase negative such as Escherichia coli O157 will not be detected.

β-glucuronidase positive Escherichia coli are bacteria that form typical blue colonies on tryptone-bile-glucuronide medium (TBX) at 16649°C under the conditions specified in this part of ISO 44.

EUROLAB assists manufacturers with ISO 16649-2 test compliance. Our test experts, with their professional working mission and principles, provide you, our manufacturers and suppliers, the best service and controlled testing process in our laboratories. Thanks to these services, businesses receive more effective, high-performance and quality testing services and provide safe, fast and uninterrupted service to their customers.

Get Offer Now

To get an appointment, to get more detailed information or to request an evaluation, you can ask us to fill in our form and reach you.

WhatsApp