High Performance Liquid Chromatography (HPLC) Test Laboratory

High performance liquid chromatography (HPLC) is a column chromatography that pumps a sample mixture or analyte in a solvent (mobile phase) at high pressure through a column containing chromatographic packaging material (stationary phase).

In this method, the sample is carried by a moving helium or nitrogen gas stream. With the high-performance liquid chromatography technique, it is possible to separate and identify compounds found in any sample that is soluble in a liquid at trace concentrations as low as parts per trillion. Thanks to its versatility, this technique is used in a variety of industrial and scientific applications such as pharmaceutical, environmental, forensics and chemistry.

The sample retention time varies depending on the interaction between the stationary phase, the analyzed molecules and the solvent or solvents used. As the sample passes through the column, it interacts at different rates between the two phases, mainly due to the different polarities in the analytes. Analytes that have the least amount of interaction with the stationary phase or the greatest amount of interaction with the mobile phase exit the column faster.

The main components in a high performance liquid chromatography (HPLC) system are: solvent reservoir or multiple reservoirs, a high pressure pump, column, injector system and detector.

Simply put, high performance liquid chromatography (HPLC) is an analytical technique for separating the components of an organic mixture that is non-volatile, thermally unstable and has relatively high molecular weights. In this method, a liquid carrier stream called mobile phase carries the injected sample through the separation column and to the detector. In the separation column, individual components are separated according to physicochemical interactions. The separated components are detected by the detector depending on the absorption of light or changes in the refractive index, electrochemical or conductivity changes, or simply the size distribution of the separated molecules.

The detector output is monitored and evaluated by the operating software, which not only makes the necessary calculations, but also supports injection volume, injection sequence, detection wavelength, wash cycles and similar operating parameters. The advanced features of the software used today have made these processes extremely user-friendly. Most of the time spent by a user is mobile stage preparation, preparation of buffers and standards, and registration entries.

Separation sciences have greatly contributed to the growth and understanding of analytical chemistry and controls for the quality of consumer products such as pharmaceuticals, food and beverages, polymers, environmental monitoring. This is especially due to chromatographic techniques and high performance liquid chromatography (HPLC).

Chromatography in general, and high performance liquid chromatography (HPLC) in particular, makes notable contributions to research and product development. High performance liquid chromatography finds wide application in pharmaceuticals, foods, biochemical research, synthetic polymers, biomolecular studies and environmental monitoring. The scope of this method is increasing at a surprising rate. High performance liquid chromatography (HPLC), a popular technique in modern laboratories, continues to gain wide acceptance.

This technique makes it possible to analyze a sample in a wide range of concentrations and molecular weights. Analyzes are traditionally performed using different column sizes and operating modes for sample quantities ranging from picograms to gram-level amounts. Advances in the field of synthetic polymers and large biomolecules have resulted from the capabilities of this method.

High performance liquid chromatography (HPLC) is the form of liquid chromatography generally used in the pharmaceutical industry, as it provides the exact results required. The results are used to quantitatively and qualitatively analyze the finished pharmaceutical products and ingredients in the manufacturing process. This technique is used to separate, quantify and define components in a mixture. HPLC technique is also used to reveal the identity of a drug and to monitor the progress of treatment on a disease. It was previously expected to be used as a complementary method for gas chromatography, but the pharmaceutical industry now uses the HPLC technique only as a chromatographic technique. One of the main benefits of this technique is its ability to explain the structure and quantify impurities in pharmaceutical formulations. This technique is particularly suitable for compounds that are not readily volatile, thermally unstable and have high molecular weights.

Our organization provides high performance liquid chromatography (HPLC) test test laboratory services to the demanding enterprises within the framework of national and international standards, with a trained and expert staff and advanced technological equipment, among numerous testing, measurement, analysis and evaluation studies.